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roche light cycler 96  (Roche)


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    Structured Review

    Roche roche light cycler 96
    Roche Light Cycler 96, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/roche+light+cycler+96/LightCycler+480+System/pmc12881754-60-12-16
    Average 99 stars, based on 6 article reviews
    roche light cycler 96 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: CXCL9 and CXCL10 Induce Expression of Nociceptive Ion Channels in Primary Sensory Neurons in Models of HIV-Associated Distal Sensory Polyneuropathy
    Article Snippet: Following the application of the detection antibody cocktail, Streptavidin-HRP was substituted for IRDye 800CW Streptavidin (1:2000) (Li-Cor, Lincoln, NE, USA) and imaged using an Odyssey CLx imaging system.

    Real-time Polymerase Chain Reaction:

    Article Title: TLR5-mediated pathogenic bacteria defense and functional maintenance of Misgurnus anguillicaudatus ovary
    Article Snippet: Pathogenic microorganisms widely present in aquatic environments significantly increase the risk of disease outbreaks in fish farming.. Toll-like receptors (TLRs) are crucial components of the innate immune system and play a vital role in resisting pathogenic microbial infections.. However, the potential functions of TLRs in the innate immune regulation of fish reproductive system remains largely unexplored.

    Article Title: Disrupted inhibin B synthesis due to Sox9 hyper-palmitoylation in Sertoli cells impairs spermatogenesis via paracrine effects.
    Article Snippet: The cells were subjected to total RNA extraction using the Total RNA Isolation Kit (082001, BEI-BEI Biotech, Zhengzhou, China), and cDNA was generated from total RNA by utilizing the PrimeScript RT reagent Kit with gDNA Eraser (RR047A, TaKaRa, Beijing, China). .. The cDNA was employed for quantitative PCR using SYBR Green Premix Pro Taq HS qPCR tracking kit (AG11733, Accurate Biotechnology Company, Changsha, China), and a Roche Light Cycler 96 Real-time PCR System (Roche Diagnostics, Switzerland) was used for fluorescence signal monitoring. ..

    Article Title: Rare TRAF5 coding variants in systemic lupus erythematosus patients aggravate pulmonary hypertension via endothelial dysfunction
    Article Snippet: Total RNA was purified from blood, cells, or tissues using Sepasol RNA I Super G (QIAGEN PAXgene Blood RNA kit, Japan), and cDNA was synthesized with qPCR RT Master Mix (Esunbio, China). .. Quantitative PCR (qPCR) was performed using the SYBR Green method on a Roche Light Cycler 96 (Roche Diagnostics), with ACTB and GAPDH as internal controls. .. TRAF5 constructs included wild-type 5′ UTR-3FLAG- NM_004619.4 (c.218+1G)-WT, variant c.218+1T (5′ UTR-3FLAG- NM_004619.4 (c.218+1T)-MUT), and variant D174fs∗5 (3FLAG- NM_004619.4 (D174fs∗5)), generated by GeneChem (Shanghai, China).

    Article Title: Mesoderm and myogenesis-related lncRNAs as Potential Markers of Myogenic Differentiation of Control and miR145 or miR181 Stimulated Mouse Pluripotent Stem Cells
    Article Snippet: .. Specific primers for lncRNAs included in the RA930A-1 LncRNA Profiler RT-qPCR Array Kit (System Biosciences) or listed in Table as well as Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher) were used for analysis in Roche Light Cycler 96 using the following program: (a) 2 min − 50°C; (b) 10 min − 95°C; (c) 15 s − 95°C (50 cycles), followed by (d) 1 min − 60°C for lncRNAs analyzed using the RA930A-1 LncRNA Profiler RT-qPCR Array Kit (System Biosciences) or (e) 30 s – selected temperature (Table ) and (f) 30 s − 72°C for other experiments. .. Data were analyzed using StepOne Software (Applied Biosystems).

    Reverse Transcription:

    Article Title: CXCL9 and CXCL10 Induce Expression of Nociceptive Ion Channels in Primary Sensory Neurons in Models of HIV-Associated Distal Sensory Polyneuropathy
    Article Snippet: A total of 50 ng of sample RNA per reaction was used with a Luna ® Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). .. Reverse transcription and PCR were performed using a Roche Light cycler 96 according to the Luna Universal Probe One-Step RT-qPCR Kit protocol. .. Primer probe sequences for TRPV1 , TRPA1 , and Beta-actin (Integrated DNA Technologies, Coralville, IA, USA) are detailed in .

    Polymerase Chain Reaction:

    Article Title: CXCL9 and CXCL10 Induce Expression of Nociceptive Ion Channels in Primary Sensory Neurons in Models of HIV-Associated Distal Sensory Polyneuropathy
    Article Snippet: A total of 50 ng of sample RNA per reaction was used with a Luna ® Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). .. Reverse transcription and PCR were performed using a Roche Light cycler 96 according to the Luna Universal Probe One-Step RT-qPCR Kit protocol. .. Primer probe sequences for TRPV1 , TRPA1 , and Beta-actin (Integrated DNA Technologies, Coralville, IA, USA) are detailed in .

    Quantitative RT-PCR:

    Article Title: CXCL9 and CXCL10 Induce Expression of Nociceptive Ion Channels in Primary Sensory Neurons in Models of HIV-Associated Distal Sensory Polyneuropathy
    Article Snippet: A total of 50 ng of sample RNA per reaction was used with a Luna ® Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA). .. Reverse transcription and PCR were performed using a Roche Light cycler 96 according to the Luna Universal Probe One-Step RT-qPCR Kit protocol. .. Primer probe sequences for TRPV1 , TRPA1 , and Beta-actin (Integrated DNA Technologies, Coralville, IA, USA) are detailed in .

    Article Title: Mesoderm and myogenesis-related lncRNAs as Potential Markers of Myogenic Differentiation of Control and miR145 or miR181 Stimulated Mouse Pluripotent Stem Cells
    Article Snippet: .. Specific primers for lncRNAs included in the RA930A-1 LncRNA Profiler RT-qPCR Array Kit (System Biosciences) or listed in Table as well as Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher) were used for analysis in Roche Light Cycler 96 using the following program: (a) 2 min − 50°C; (b) 10 min − 95°C; (c) 15 s − 95°C (50 cycles), followed by (d) 1 min − 60°C for lncRNAs analyzed using the RA930A-1 LncRNA Profiler RT-qPCR Array Kit (System Biosciences) or (e) 30 s – selected temperature (Table ) and (f) 30 s − 72°C for other experiments. .. Data were analyzed using StepOne Software (Applied Biosystems).

    SYBR Green Assay:

    Article Title: Disrupted inhibin B synthesis due to Sox9 hyper-palmitoylation in Sertoli cells impairs spermatogenesis via paracrine effects.
    Article Snippet: The cells were subjected to total RNA extraction using the Total RNA Isolation Kit (082001, BEI-BEI Biotech, Zhengzhou, China), and cDNA was generated from total RNA by utilizing the PrimeScript RT reagent Kit with gDNA Eraser (RR047A, TaKaRa, Beijing, China). .. The cDNA was employed for quantitative PCR using SYBR Green Premix Pro Taq HS qPCR tracking kit (AG11733, Accurate Biotechnology Company, Changsha, China), and a Roche Light Cycler 96 Real-time PCR System (Roche Diagnostics, Switzerland) was used for fluorescence signal monitoring. ..

    Article Title: Rare TRAF5 coding variants in systemic lupus erythematosus patients aggravate pulmonary hypertension via endothelial dysfunction
    Article Snippet: Total RNA was purified from blood, cells, or tissues using Sepasol RNA I Super G (QIAGEN PAXgene Blood RNA kit, Japan), and cDNA was synthesized with qPCR RT Master Mix (Esunbio, China). .. Quantitative PCR (qPCR) was performed using the SYBR Green method on a Roche Light Cycler 96 (Roche Diagnostics), with ACTB and GAPDH as internal controls. .. TRAF5 constructs included wild-type 5′ UTR-3FLAG- NM_004619.4 (c.218+1G)-WT, variant c.218+1T (5′ UTR-3FLAG- NM_004619.4 (c.218+1T)-MUT), and variant D174fs∗5 (3FLAG- NM_004619.4 (D174fs∗5)), generated by GeneChem (Shanghai, China).

    Fluorescence:

    Article Title: Disrupted inhibin B synthesis due to Sox9 hyper-palmitoylation in Sertoli cells impairs spermatogenesis via paracrine effects.
    Article Snippet: The cells were subjected to total RNA extraction using the Total RNA Isolation Kit (082001, BEI-BEI Biotech, Zhengzhou, China), and cDNA was generated from total RNA by utilizing the PrimeScript RT reagent Kit with gDNA Eraser (RR047A, TaKaRa, Beijing, China). .. The cDNA was employed for quantitative PCR using SYBR Green Premix Pro Taq HS qPCR tracking kit (AG11733, Accurate Biotechnology Company, Changsha, China), and a Roche Light Cycler 96 Real-time PCR System (Roche Diagnostics, Switzerland) was used for fluorescence signal monitoring. ..



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